All-Star Mode is unlocked when all characters are unlocked or you have finished Emissary of Darkness. Like in SSBN, the player faces characters via their series. The order of the fights are ordered by the release date. After completing All-Star Mode, you will obtain the Final Smash trophy of that character.
| Universe | Year |
Characters Fought |
|---|---|---|
| Game & Watch | 1980 | Mr. Game & Watch |
| Mario | 1981 |
Mario Luigi Peach Bowser Bowser Jr./Shadow Mario |
| Donkey Kong | 1981 |
Donkey Kong Diddy Kong King K. Rool |
| Ice Climber | 1984 | Ice Climbers |
| R.O.B. | 1985 | R.O.B. |
| Legend of Zelda | 1986 |
Link Zelda/Sheik Ganondorf Toon Link Toon Zelda/Sheik |
| Nazo no Murasame-jo | 1986 | Takamaru |
| Metroid | 1986 |
Samus/Zero Suit Samus Ridley |
| Kid Icarus | 1986 |
Pit Palutena |
| Metal Gear | 1987 |
Snake Raiden |
| EarthBound | 1989 |
Ness Lucas Poo |
| Fire Emblem | 1990 |
Marth Ike Roy Black Knight |
| F-Zero | 1990 |
Captain Falcon Black Shadow |
| Yoshi | 1990 |
Yoshi Birdo |
| Sonic | 1991 |
Sonic Shadow |
| Kirby | 1992 |
Kirby Meta Knight King Dedede Galacta Knight Bandana Dee |
| Wario | 1992 |
Wario Waluigi |
| Star Fox | 1993 |
Fox Falco Wolf Krystal Panther Caroso |
| Pokemon | 1996 |
Pikachu Zoroark Mewtwo Jigglypuff Pokemon Trainer |
| Sin and Punishment | 2000 | Saki |
| Golden Sun | 2001 |
Isaac Matthew |
| Pikmin | 2001 |
Olimar Louie |
| Chibi-Robo! | 2005 | Chibi-Robo |