The Mario Family is a project attempting to connect every single possible family member of the Mario Family into a Family Tree. This includes all fanon characters. For the sake of simplicity, we will not be counting other media relatives unless they exist on this wiki.
Parents [ ]
Mario's original parents, Giovanni and Giovanna. (As depicted in the Yoshi Island 4koma series)
Mario and Luigi's adoptive parents, Steven and Mary (during their time in Brooklyn)
Generation Mario [ ]
Mario (canon son of Mario's Original Parents)
Luigi (canon son of Mario's Original Parents)
Naluigi (fanon son of Mario's Original Parents, got lost)
Wario (thought to be the canon nephew of Mario's Original Parents)
Waluigi (thought to be the canon nephew of Mario's Original Parents)
Maria (niece of Mario and Luigi's Original Parents)
Luise (niece of Mario and Luigi's Original Parents)
Guido Mario (fanon cousin of Mario and Luigi, unknown parentage)
Walice (fanon 2nd niece of Mario and Luigi's original parents)
Waria (fanon 2nd niece of Mario and Luigi's original parents)
Eario (completely unknown relationship to the families)
Liu (Mario and Luigi's brother, likely Original Parents)
Malgeet (Mario and Luigi's brother, likely Original Parents)
Lugeet (Mario and Luigi's brother, likely Original Parents)
Tony Mario (Mario's second cousin, unknown parentage)
Ignacio (nephew of Mario and Luigi's adopted family)
Baluigi (younger brother of Waluigi)
Malleo (Mario and Luigi's stupid cousin)
Dr. Mario (Cousin with unknown connections)
Dr. Luigi (Cousin with unknown connections)
Dr. Wario (Cousin with unknown connections)
Dr. Waluigi (Cousin with unknown connections)
Generation 2 [ ]
The children of the Mario Generation.
Antonio (nephew of Mario and Luigi, unknown parents)
Tram Vulpes Jr (3rd Cousin, Once Removed in the Brooklyn Line)
Pauline Vulpes (3rd Cousin, Once Removed in the Brooklyn Line)
Idolum Tergum (3rd Cousin, Once Removed in the Brooklyn Line)
Mary Tergum (3rd Cousin, Once Removed in the Brooklyn Line)
Susie Tergum (3rd Cousin, Once Removed in the Brooklyn Line)
Louisa Tergum (3rd Cousin, Once Removed in the Brooklyn Line)
Aka-Chan (3rd Cousin, Once Removed in the Brooklyn Line)
Ao-Kun (3rd Cousin, Once Removed in the Brooklyn Line)
Kii-Yan (3rd Cousin, Once Removed in the Brooklyn Line)
No Biological Connection [ ]
No biological connections but are still considered part of the Mario Family.
Jen (honorary Mario Sister, likely not connected to family at all)
Willy (honorary Mario brother, likely not connected to family at all)
Ram (evil clone, brother of Uil)
Uil (evil clone, brother of Ram)
Negative Mario (Negative clone of Mario)
Antonio (Mario's Alternate Dimension Self)
Carlo (Luigi's Alternate Dimension Self)
Paramario (parallel version to Mario from the Flower Kingdom)
Balugio (Unknown Connection)
[1] Marthy (Mario in the Mirror Dimension)
Louis (Luigi in the Mirror Dimension)
Goody (Wario in the Mirror Dimension)
Goodice (Waluigi in the Mirror Dimension)
Maryotto (Maria from the Mirror Dimension)
Louise G. (Luise in the Mirror World)
Goodotto (Waria in the Mirror Dimension)
Goodice G. (Walice in the Mirror Dimension)
Questions [ ]
Are Jumpman and Pauline Mario and Luigi's original parents?
The pages seemingly contradict each other in this case although this can be changed if people feel it's necessary.
Is Guido a cousin of Mario's adoptive parents or original parents?
The fact he was running around in Brooklyn suggests he was cousins with Mario's original parents, although unless the creator states one way or the other, it's left unknown.
Time to clear this up once and for all: are Wario and Waluigi cousins of Mario and Luigi?
Likewise, are Wario and Waluigi related to each other?